Abstract:
complex study of the aerial part of Serratula coronata L. cultivated in the collection area of medicinal
plants of the IRPH «Phytochemistry» (Karaganda) in different phases of growth and using the most optimal
extraction methods has been carried out. The content of the main active component of ecdysterone (20E)has
been studied. Investigation of the seasonal dynamics of ecdysterone distribution under conditions of varying
extraction methods shows that its maximum accumulation is observed during the vegetative phase, and the
optimal method in this phase is extraction with isobutyl alcohol, the extract of which contains 13.86 % of
ecdysterone and the maceration method with 96.2 % ethyl alcohol with a 20E content of 12.03 %, respectively.
It is shown that the maceration with 96.2 % ethyl alcohol is technologically optimal, which fully complies
with the international standards of good manufacturing practice (GMP) under pharmaceutical production
conditions and excludes the use of toxic and expensive isobutyl alcohol solvent. It has been found that the
content of ecdysterone from the beginning of vegetation to the final phase goes down, which is confirmed by
the data of high-performance liquid chromatography (HPLC). It is assumed that there is an outflow of
ecdysterone to the root system, and then its redistribution occurs as the plant develops further with a partial
discharge into the soil. Based on the data on the quantitative content of the target component by the HPLC
method, it is recommended that for the preparation of the ecdysterone substance of many actoprotective
phytopreparations and valuable WS, the preparation of the above-ground biomass of Serratula coronata L.
should be carried out during the vegetation phase of this taxon.
