Authors showed that intravenous injection amino acids Cystein and L-Hystidine prevent in majority of animals
developing of experimental diabetes caused by chemicals formed in B-cells chelat complexes with
Zn+2-ions. It was shown that in all cases administration of these amino acids prior injection of diabetogenic
chelator as Diphe nylthiocarbazon (Dithizon) accompanied by almost complete absence of binding of
Zn+2-ions in В-cells with formation of toxic complexes which result destruction and death of B-cells within
short time. Authors suppose that protective effect is determined by presence in molecules of Cystein and
L-Hystidine of SH-groups reacted with reacted with Zn+2-ions in B-cells with forming of not toxic complexes
and not able to forming of complexes with a Dithizon. Authors showed also that from the used various
histochemical and immunocytohistochemical methods insulin staining in B-cell more precise results was obtained
using of immunohistochemical and fluorescent Diethylpseudoisocyanine methods. However, Aldehyde-
fuchsine method and method Victoria 4R more suitable for investigation state of histostructure of pancreatic
islets.
