Abstract:
Authors investigated preventive effect of Reduced form of Glutathione on developing of diabetes caused by
diabetogenic zinc binding ligands. It is shown that only Reduced form of Glutathione contains SH-radical in
chemical structure possesses ability to protect B-cells of death unlike the Oxidized form of the Glutathione,
free of it. It was established that injection of R-Glutathione, 1000 mg/kg completely connects with zinc in
B-cells that protect cells of formation of toxic complexes causing destruction of B-cells and development of
diabetes. Investigation of chemical aspect of interaction of R-Glutathione with zinc showed that administration
of it result a complete blocking of zinc in B-cells therefore zinc does not revealed using of high specific
and sensitive histochemical methods. The studying of duration of blocking of zinc in B-cells showed that
complex Zn-R-Glutathione begins to dissociate with partial release of zinc approximately about 36 hours after
injection and 48 hours later majority of complex is dissociate. Authors suppose that preventive ability of
R-Glutathione is caused by ability to formation of not toxic temporary complex with zinc; zinc atom is fixed
between atom of sulfur of the SH-radical and atom of oxygen or nitrogen which are contained in a molecule
of R-Glutathione as well as after administration of diabetogenic chelators between of sulfur atom — and either
oxygen atom, or atoms of nitrogen or carbon on the other hand.
